Confocal microscopy protocol for adherent cells. . Confocal microscopy removes t...
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Confocal microscopy protocol for adherent cells. . Confocal microscopy removes the out-of-focus light from your image by sending all the light coming from your specimen through a tiny pinhole through which only in-focus light can pass. A key feature is a small aperture, or pinhole, positioned in front of the detector. Learn how confocal microscopes eliminate out-of-focus light for sharper images. His original idea was to move the sample in order to scan it, rather than to use scanning mirrors. In contrast, a confocal microscope uses point illumination (see Point Spread Function) and a pinhole in an optically conjugate plane in front of the detector to eliminate out-of-focus signal – the name "confocal" stems from this configuration. The confocal microscope was invented by Marvin Minsky, who has written a nice summary about this on the web. Covers pinhole aperture, optical sectioning, z-stack acquisition, and biological applications. The Laser Scanning Confocal Microscope (LSCM, or confocal) is a specialized type of light microscope. In contrast, a confocal microscope uses point illumination (see Point Spread Function) and a pinhole in an optically conjugate plane in front of the detector to eliminate out-of-focus signal – the name "confocal" stems from this configuration.
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